Institute of Cell Biology

Alumni

Prof. Daniel Schümperli

mRNA processing and gene therapy

I retired from the University of Bern at the end of July 2016. One of our recent activities dealing with alternative splicing in the human hereditary disease Erythropoietic Protoporphyria is persued by the group of Prof. Jonathan Hall at ETHZ, and I continue to act as an external advisor for this project. Moreover, I remain in contact with the national research excellence network NCCR RNA and Disease of the Swiss National Science Foundation which has strongly supported our research over the past few years, both financially and scientifically.

Research focus

During my career as independent scientist, my collaborators and myself have been investigating basic mechanisms active in the expression of genetic information. The genes coding for proteins are first copied into primary RNA transcripts which then need to be processed in various ways. A major focus of our research has been the formation of mRNA 3' ends which requires an endonucleolytic cleavage of the mRNA precursor. For the bulk of mRNAs, the cleavage product is then extended with a polyA tail which protects the mRNA from degradation and allows its translation. A special case are the mRNAs coding for histone proteins. In animals and humans, these are encoded by genes without introns, and the 3' ends are generated by a distinct mechanism in which they do not acquire a polyA tail. My group has worked on both of these 3' end processing reactions, but most intensely on histone RNA processing.

These 3' end processing reactions are highly regulated. Alternative polyA site selection can affect the structure and function of the protein encoded by the gene in question, or it can add or remove regulatory sequences from the mRNA that control its stability, translation or subcellular localization. Moreover, histone RNA 3' end processing is highly regulated during the cell cycle to ensure that histone proteins are synthesized primarily during S phase when the cellular DNA is replicated. These regulations can fail which is an important cause of inherited disorders and also contributes to some acquired diseases such as cancers.

The second process that has interested us more from a medical point of view is the removal of non-coding sequences (introns) from a primary transcript by the process called splicing. Higher organisms, and in particular humans, make extensive use of alternative splicing to produce different proteins from a single gene. Alternative splicing is often regulated and contributes strongly to developmental processes. Moreover, many of the mutations responsible for inherited disorders affect the splicing of the affected gene and thereby lead to the production of a faulty protein or insufficient amounts of the correct one. Over the years, our basic studies on a small nuclear ribonucleoprotein particle involved in histone RNA processing (the U7 snRNP) have allowed us to derive modified versions of this particle that can be used to modulate alternative splicing. We have shown that these tools can be used for the treatment of certain inherited diseases such as the fatal neuromuscular disease Spinal Muscular Atrophy (SMA) and a metabolic disorder that causes a very painful light sensitivity, Erythropoietic Protoporphyria (EPP).

Publications

Jump to: Journal Article | Book Section | Conference or Workshop Item
Number of items: 79.

Journal Article

Halloy, François; Brönnimann, Paulina; Hall, Jonathan; Schümperli, Daniel (2022). Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals. Methods in molecular biology, 2537, pp. 335-350. Springer 10.1007/978-1-0716-2521-7_19

Barman-Aksözen, Jasmin; Ćwiek, Paulina; Bansode, Vijay B.; Koentgen, Frank; Trüb, Judith; Pelczar, Pawel; Cinelli, Paolo; Schneider-Yin, Xiaoye; Schümperli, Daniel; Minder, Elisabeth I. (2017). Modeling the ferrochelatase c.315-48C modifier mutation for erythropoietic protoporphyria (EPP) in mice. Disease models & mechanisms, 10(3), pp. 225-233. Company of Biologists Ltd. 10.1242/dmm.027755

Li, Meiling; Lightfoot, Helen Louise; Halloy, Francois; Malinowska, Anna; Berk, Christian; Behera, Alok; Schümperli, Daniel; Hall, Jonathan (2017). Synthesis and cellular activity of stereochemically-pure 2’-O-(2-methoxyethyl)-phosphorothioate oligonucleotides. Chemical communications, 53(3), pp. 541-544. Royal Society of Chemistry 10.1039/C6CC08473G

Odermatt, Philipp; Trüb, Judith; Furrer, Lavinia; Fricker, Roger; Marti, Andreas; Schümperli, Daniel (2016). Somatic therapy of a mouse SMA model with a U7 snRNA gene correcting SMN2 splicing. Molecular therapy, 24(10), pp. 1797-1805. Nature Publishing Group 10.1038/mt.2016.152

Schümperli, Daniel (2016). Rudolf Weber (1922-2015): a driving force in the transition of developmental biology into a molecular and cellular science. Development genes and evolution, 226(2), pp. 65-67. Springer 10.1007/s00427-016-0532-9

Romeo, Valentina; Schümperli, Daniel (2016). Cycling in the nucleus: regulation of RNA 3' processing and nuclear organization of replication-dependent histone genes. Current opinion in cell biology, 40, pp. 23-31. Elsevier 10.1016/j.ceb.2016.01.015

Neve, Anuja Vilas; Trüb, Judith; Saxena, Smita; Schümperli, Daniel (2016). Central and peripheral defects in motor units of the diaphragm of spinal muscular atrophy mice. Molecular and cellular neuroscience, 70, pp. 30-41. Elsevier 10.1016/j.mcn.2015.11.007

Duque, Sandra I; Arnold, W David; Odermatt, Philipp; Li, Xiaohui; Porensky, Paul N; Schmelzer, Leah; Meyer, Kathrin; Kolb, Stephen J; Schümperli, Daniel; Kaspar, Brian K; Burghes, Arthur H M (2015). A large animal model of spinal muscular atrophy and correction of phenotype. Annals of neurology, 77(3), pp. 399-414. Wiley-Blackwell 10.1002/ana.24332

Raczynska, Katarzyna Dorota; Ruepp, Marc-David; Brzek, Aleksandra; Reber, Stefan; Romeo, Valentina; Rindlisbacher, Barbara; Heller, Manfred; Szweykowska-Kulinska, Zofia; Jarmolowski, Artur; Schümperli, Daniel (2015). FUS/TLS contributes to replication-dependent histone gene expression by interaction with U7 snRNPs and histone-specific transcription factors. Nucleic acids research, 43(20), pp. 9711-9728. Oxford University Press 10.1093/nar/gkv794

Romeo, Valentina; Griesbach, Esther; Schümperli, Daniel (2014). CstF64: cell cycle regulation and functional role in 3' end processing of replication-dependent histone mRNAs. Molecular and cellular biology, 34(23), pp. 4272-4284. American Society for Microbiology 10.1128/MCB.00791-14

Voigt, Tilman; Neve, Anuja Vilas; Schümperli, Daniel (2014). The craniosacral progression of muscle development influences the emergence of neuromuscular junction alterations in a severe murine model for spinal muscular atrophy. Neuropathology and Applied Neurobiology, 40(4), pp. 416-434. Wiley 10.1111/nan.12064

Huo, Qing; Kayikci, Melis; Odermatt, Philipp; Meyer, Kathrin; Michels, Olivia; Saxena, Smita; Ule, Jernej; Schümperli, Daniel (2014). Splicing changes in SMA mouse motoneurons and SMN-depleted neuroblastoma cells: evidence for involvement of splicing regulatory proteins. RNA biology, 11(11), pp. 1430-1446. Landes Bioscience 10.1080/15476286.2014.996494

Ruepp, Marc-David; Schweingruber, Christoph; Kleinschmidt, Nicole; Schümperli, Daniel (2011). Interactions of CstF-64, CstF-77, and symplekin: Implications on localisation and function. Molecular biology of the cell, 22(1), pp. 91-104. Bethesda, Md.: American Society for Cell Biology 10.1091/mbc.E10-06-0543

Ruepp, Marc-David; Schümperli, Daniel; Barabino, Silvia M.L. (2011). mRNA 3' end processing and more - multiple functions of mammalian cleavage factor I-68. RNA - a publication of the RNA Society, 2(1), pp. 79-91. Woodbury, N.Y.: Cold Spring Harbor Laboratory Press 10.1002/wrna.35

Voigt, Tilman; Meyer, Kathrin; Baum, Oliver; Schümperli, Daniel (2010). Ultrastructural changes in diaphragm neuromuscular junctions in a severe mouse model for Spinal Muscular Atrophy and their prevention by bifunctional U7 snRNA correcting SMN2 splicing. Neuromuscular disorders, 20(11), pp. 744-52. Amsterdam: Elsevier 10.1016/j.nmd.2010.06.010

Matulis, Gediminas; Sanderson, Joseph P; Lissin, Nikolai M; Asparuhova, Maria B; Bommineni, Gopal R; Schümperli, Daniel; Schmidt, Richard R; Villiger, Peter M; Jakobsen, Bent K; Gadola, Stephan D (2010). Innate-like control of human iNKT cell autoreactivity via the hypervariable CDR3beta loop. PLoS biology, 8(6), e1000402. Lawrence, Kans.: Public Library of Science 10.1371/journal.pbio.1000402

Ruepp, Marc-David; Vivarelli, Silvia; Pillai, Ramesh S.; Kleinschmidt, Nicole; Azzouz, Teldja N.; Barabino, Silvia M.L.; Schümperli, Daniel (2010). The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 30-end processing of animal histone mRNAs. Nucleic acids research, 38(21), pp. 7637-7650. London: Oxford University Press 10.1093/nar/gkq613

Ittig, Damian; Luisier, Samuel; Weiler, Jan; Schümperli, Daniel; Leumann, Christian J. (2010). Improving gene silencing of siRNAs via tricyclo-DNA modification. Artificial DNA, PNA & XNA, 1(1), pp. 9-16. Austin, Tex.: Landes Bioscience 10.4161/adna.1.1.11385

Nlend, Rachel Nlend; Meyer, Kathrin; Schümperli, Daniel (2010). Repair of pre-mRNA splicing: Prospects for a therapy for Spinal Muscular Atrophy. RNA biology, 7(4), pp. 730-740. Georgetown, Tex.: Landes Bioscience 10.4161/rna.7.4.12206

Barta, Andrea; Schümperli, Daniel (2010). Editorial on Alternative Splicing and Disease. RNA - a publication of the RNA Society, 7(4), pp. 388-389. Woodbury, N.Y.: Cold Spring Harbor Laboratory Press 10.4161/rna.7.4.12818

Meyer, Kathrin; Marquis, Julien; Trüb, Judith; Nlend Nlend, Rachel; Verp, Sonia; Ruepp, Marc-David; Imboden, Hans; Barde, Isabelle; Trono, Didier; Schümperli, Daniel (2009). Rescue of a severe mouse model for spinal muscular atrophy by U7 snRNA-mediated splicing modulation. Human molecular genetics, 18(3), pp. 546-555. Oxford: Oxford University Press 10.1093/hmg/ddn382

Marquis, J.; Kampfer, S.S.; Angehrn, L.; Schümperli, Daniel (2009). Doxycycline-controlled splicing modulation by regulated antisense U7 snRNA expression cassettes. Gene therapy, 16(1), pp. 70-7. London: Nature Publishing Group 10.1038/gt.2008.138

Ruepp, Marc-David; Aringhieri, Chiara; Vivarelli, Silvia; Cardinale, Stefano; Paro, Simona; Schümperli, Daniel; Barabino, Silvia M.L. (2009). Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export. Molecular biology of the cell, 20(24), pp. 5211-23. Bethesda, Md.: American Society for Cell Biology 10.1091/mbc.E09-05-0389

Asparuhova, Maria B.; Barde, Isabelle; Trono, Didier; Schranz, Karin; Schümperli, Daniel (2008). Development and characterization of a triple combination gene therapy vector inhibiting HIV-1 multiplication. Journal of gene medicine, 10(10), pp. 1059-1070. Chichester: Wiley 10.1002/jgm.1238

Marquis, Julien; Meyer, Kathrin; Angehrn, Larissa; Kämpfer, Sacha; Rothen-Rutishauser, Barbara; Schümperli, Daniel (2007). Spinal muscular atrophy: SMN2 pre-mRNA splicing corrected by a U7 snRNA derivative carrying a splicing enhancer sequence. Molecular therapy, 15(8), pp. 1479-1486. New York, N.Y.: Nature Publishing Group 10.1038/sj.mt.6300200

Asparuhova, Maria B.; Marti, Gabriela; Liu, Songkai; Serhan, Fatima; Trono, Didier; Schümperli, Daniel (2007). Inhibition of HIV-1 multiplication by a modified U7 snRNA inducing Tat and Rev exon skipping. Journal of gene medicine, 9(5), pp. 323-334. Chichester: Wiley 10.1002/jgm.1027

Scholl, Raphael; Marquis, Julien; Meyer, Kathrin; Schümperli, Daniel (2007). Spinal Muscular Atrophy: position and functional importance of the branch site preceding SMN exon 7. RNA biology, 4(1), pp. 34-37. Georgetown, Tex.: Landes Bioscience 10.4161/rna.4.1.4534

Azzouz, Teldja N.; Pillai, Ramesh S.; Däpp, Christoph; Chari, Ashwin; Meister, Gunter; Kambach, Christian; Fischer, Utz; Schümperli, Daniel (2005). Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes. Journal of biological chemistry, 280(41), pp. 34435-34440. American Society for Biochemistry and Molecular Biology 10.1074/jbc.M505077200

Xu, Hongzhi; Pillai, Ramesh S.; Azzouz, Teldja N.; Shpargel, Karl B.; Kambach, Christian; Hebert, Michael D.; Schümperli, Daniel; Matera, A. Gregory (2005). The C-terminal domain of coilin interacts with Sm proteins and U snRNPs. Chromosoma, 114(3), pp. 155-166. Springer 10.1007/s00412-005-0003-y

Gorgoni, Barbara; Andrews, Stuart; Schaller, André; Schümperli, Daniel; Gray, Nicola K; Müller, Berndt (2005). The stem-loop binding protein stimulates histone translation at an early step in the initiation pathway. RNA - a publication of the RNA Society, 11(7), pp. 1030-1042. Woodbury, N.Y.: Cold Spring Harbor Laboratory Press 10.1261/rna.7281305

Azzouz, Teldja N.; Gruber, Andreas; Schümperli, Daniel (2005). U7 snRNP-specific Lsm11 protein: dual binding contacts with the 100 kDa zinc finger processing factor (ZFP100) and a ZFP100-independent function in histone RNA 3' end processing. Nucleic acids research, 33(7), pp. 2106-2117. Oxford University Press 10.1093/nar/gki516

Ittig, D.; Liu, Shi-Xia; Renneberg, Dorte; Schümperli, Daniel; Leumann, Christian (2004). Nuclear antisense effects in cyclophilin A pre-mRNA splicing by oligonucleotides: A comparison of tricyclo-DNA with LNA. Nucleic acids research, 32(1), pp. 346-353. Oxford University Press 10.1093/nar/gkh187

Liu, Songkai; Asparuhova, Maria; Brondani, Vincent; Ziekau, Ingrid; Klimkait, Thomas; Schümperli, Daniel (2004). Inhibition of HIV-1 multiplication by antisense U7 snRNAs and siRNAs targeting cyclophilin A. Nucleic acids research, 32(12), pp. 3752-3759. Oxford University Press 10.1093/nar/gkh715

Schümperli, Daniel; Pillai, R S (2004). The special Sm core structure of the U7 snRNP: far-reaching significance of a small nuclear ribonucleoprotein. Cellular and molecular life sciences, 61(19-20), pp. 2560-2570. Birkhäuser-Verlag; www.birkhauser.ch 10.1007/s00018-004-4190-0

Asparuhova, Maria B.; Kole, Ryszard; Schümperli, Daniel (2004). Antisense derivatives of U7 and other small nuclear RNAs as tools to modify pre-mRNA splicing patterns. Gene Therapy and Regulation, 2(4), pp. 321-349. VSP International Science Publishers 10.1163/1568558043967472

Azzouz, Teldja N; Schümperli, Daniel (2003). Evolutionary conservation of the U7 small nuclear ribonucleoprotein in Drosophila melanogaster. RNA - a publication of the RNA Society, 9(12), pp. 1532-1541. Cold Spring Harbor Laboratory Press 10.1261/rna.5143303

Pillai, Ramesh S; Grimmler, Matthias; Meister, Gunter; Will, Cindy L; Lührmann, Reinhard; Fischer, Utz; Schümperli, Daniel (2003). Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN complex and the role of a new component, Lsm11, in histone RNA processing. Genes & development, 17(18), pp. 2321-2333. Cold Spring Harbor Laboratory Press 10.1101/gad.274403

Brun, C; Suter, D; Pauli, C; Dunant, P; Lochmüller, H; Burgunder, J M; Schümperli, Daniel; Weis, J (2003). U7 snRNAs induce correction of mutated dystrophin pre-mRNA by exon skipping. Cellular and molecular life sciences, 60(3), pp. 557-566. Springer

Pettitt, Jonathan; Crombie, Catriona; Schümperli, Daniel; Müller, Berndt (2002). The Caenorhabditis elegans histone hairpin-binding protein is required for core histone gene expression and is essential for embryonic and postembryonic cell division. Journal of cell science, 115(Pt 4), pp. 857-866. Company of Biologists Limited

Zanier, Katia; Luyten, Ingrid; Crombie, Catriona; Muller, Berndt; Schümperli, Daniel; Linge, Jens P; Nilges, Michael; Sattler, Michael (2002). Structure of the histone mRNA hairpin required for cell cycle regulation of histone gene expression. RNA - a publication of the RNA Society, 8(1), pp. 29-46. Cold Spring Harbor Laboratory Press

Renneberg, Dorte; Bouliong, Emilie; Reber, Ulrich; Schümperli, Daniel; Leumann, Christian J. (2002). Antisense properties of tricyclo-DNA. Nucleic acids research, 30(13), pp. 2751-2757. Oxford University Press 10.1093/nar/gkf412

Pillai, R S; Will, C L; Lührmann, R; Schümperli, Daniel; Müller, B (2001). Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein. EMBO journal, 20(19), pp. 5470-5479. Nature Publishing Group 10.1093/emboj/20.19.5470

Michel, F; Schümperli, Daniel; Müller, B (2000). Specificities of Caenorhabditis elegans and human hairpin binding proteins for the first nucleotide in the histone mRNA hairpin loop. RNA - a publication of the RNA Society, 6(11), pp. 1539-1550. Cold Spring Harbor Laboratory Press

Martin, F; Michel, F; Zenklusen, D; Müller, B; Schümperli, Daniel (2000). Positive and negative mutant selection in the human histone hairpin-binding protein using the yeast three-hybrid system. Nucleic acids research, 28(7), pp. 1594-1603. Oxford University Press 10.1093/nar/28.7.1594

Suter, Daniel; Tomasini, Reto; Reber, Ueli; Gorman, Linda; Kole, Ryszard; Schümperli, Daniel (1999). Double-target antisense U7 snRNAs promote efficient skipping of an aberrant exon in three human β-thalassemic mutations. Human molecular genetics, 8(13), pp. 2415-2423. Oxford University Press 10.1093/hmg/8.13.2415

Sierakowska, H; Sambade, M J; Schümperli, Daniel; Kole, R (1999). Sensitivity of splice sites to antisense oligonucleotides in vivo. RNA - a publication of the RNA Society, 5(3), pp. 369-377. Cold Spring Harbor Laboratory Press

Walther, T N; Wittop Koning, T H; Schümperli, Daniel; Müller, B (1998). A 5'-3' exonuclease activity involved in forming the 3' products of histone pre-mRNA processing in vitro. RNA - a publication of the RNA Society, 4(9), pp. 1034-1046. Cold Spring Harbor Laboratory Press

Gorman, L; Suter, D; Emerick, V; Schümperli, Daniel; Kole, R (1998). Stable alteration of pre-mRNA splicing patterns by modified U7 small nuclear RNAs. Proceedings of the National Academy of Sciences of the United States of America - PNAS, 95(9), pp. 4929-4934. National Academy of Sciences NAS

Furger, A; Schaller, A; Schümperli, Daniel (1998). Functional importance of conserved nucleotides at the histone RNA 3' processing site. RNA - a publication of the RNA Society, 4(3), pp. 246-256. Cold Spring Harbor Laboratory Press

Müller, B; Schümperli, Daniel (1997). The U7 snRNP and the hairpin binding protein: Key players in histone mRNA metabolism. Seminars in cell & developmental biology, 8(6), pp. 567-576. Elsevier 10.1006/scdb.1997.0182

Wyler-Duda, P; Bernard, V; Stadler, M; Suter, D; Schümperli, Daniel (1997). Histone H4 mRNA from the nematode Ascaris lumbricoides is cis-spliced and polyadenylated. Biochimica et biophysica acta (BBA) - gene structure and expression, 1350(3), pp. 259-261. Elsevier 10.1016/S0167-4781(96)00235-7

Martin, F; Schaller, A; Eglite, S; Schümperli, Daniel; Müller, B (1997). The gene for histone RNA hairpin binding protein is located on human chromosome 4 and encodes a novel type of RNA binding protein. EMBO journal, 16(4), pp. 769-778. Nature Publishing Group 10.1093/emboj/16.4.769

Gruber, Andreas; Stettler, P; Heiniger, P; Schümperli, Daniel; Lanzrein, B (1996). Polydnavirus DNA of the braconid wasp Chelonus inanitus is integrated in the wasp's genome and excised only in later pupal and adult stages of the female. Journal of general virology, 77(11), pp. 2873-2879. Society for General Microbiology 10.1099/0022-1317-77-11-2873

Turner, P C; Whalen, A; Schümperli, Daniel; Matera, A G (1996). The bona fide mouse U7 snRNA gene maps to a different chromosome than two U7 pseudogenes. Genomics, 31(2), pp. 250-252. Elsevier 10.1006/geno.1996.0042

Stefanovic, B; Wittop Koning, T H; Schümperli, Daniel (1995). A synthetic histone pre-mRNA-U7 small nuclear RNA chimera undergoing cis cleavage in the cytoplasm of Xenopus oocytes. Nucleic acids research, 23(16), pp. 3152-3160. Information Retrieval Ltd.

Stefanovic, B; Hackl, W; Lührmann, R; Schümperli, Daniel (1995). Assembly, nuclear import and function of U7 snRNPs studied by microinjection of synthetic U7 RNA into Xenopus oocytes. Nucleic acids research, 23(16), pp. 3141-3151. Information Retrieval Ltd.

Albrecht, U; Wyler, T; Pfister-Wilhelm, R; Gruber, Andreas; Stettler, P; Heiniger, P; Kurt, E; Schümperli, Daniel; Lanzrein, B (1994). Polydnavirus of the parasitic wasp Chelonus inanitus (Braconidae): characterization, genome organization and time point of replication. Journal of general virology, 75(12), pp. 3353-3363. Society for General Microbiology 10.1099/0022-1317-75-12-3353

Spycher, C; Streit, A; Stefanovic, B; Albrecht, D; Koning, T H; Schümperli, Daniel (1994). 3' end processing of mouse histone pre-mRNA: evidence for additional base-pairing between U7 snRNA and pre-mRNA. Nucleic acids research, 22(20), pp. 4023-4030. Information Retrieval Ltd.

Wittop Koning, T H; Schümperli, Daniel (1994). RNAs and ribonucleoproteins in recognition and catalysis. European journal of biochemistry, 219(1-2), pp. 25-42. Blackwell Science 10.1111/j.1432-1033.1994.tb19911.x

Streit, A; Koning, T W; Soldati, D; Melin, L; Schümperli, Daniel (1993). Variable effects of the conserved RNA hairpin element upon 3' end processing of histone pre-mRNA in vitro. Nucleic acids research, 21(7), pp. 1569-1575. Information Retrieval Ltd.

Grimm, C; Stefanovic, B; Schümperli, Daniel (1993). The low abundance of U7 snRNA is partly determined by its Sm binding site. The EMBO Journal, 12(3), pp. 1229-1238. EMBO Press

Mital, R; Albrecht, U; Schümperli, Daniel (1993). Detection of UV-induced RNA:protein crosslinks in snRNPs by oligonucleotides complementary to the snRNA. Nucleic acids research, 21(4), pp. 1049-1050. Information Retrieval Ltd.

Melin, L; Soldati, D; Mital, R; Streit, A; Schümperli, Daniel (1992). Biochemical demonstration of complex formation of histone pre-mRNA with U7 small nuclear ribonucleoprotein and hairpin binding factors. The EMBO Journal, 11(2), pp. 691-697. EMBO Press

Harris, M E; Böhni, R; Schneiderman, M H; Ramamurthy, L; Schümperli, Daniel; Marzluff, W F (1991). Regulation of histone mRNA in the unperturbed cell cycle: evidence suggesting control at two posttranscriptional steps. Molecular and cellular biology, 11(5), pp. 2416-2424. American Society for Microbiology

Gruber, Andreas; Soldati, D; Burri, M; Schümperli, Daniel (1991). Isolation of an active gene and of two pseudogenes for mouse U7 small nuclear RNA. Biochimica et biophysica acta (BBA) - gene structure and expression, 1088(1), pp. 151-154. Elsevier 10.1016/0167-4781(91)90167-K

Gruber, Andreas; Streit, A; Reist, M; Benninger, P; Böhni, R; Schümperli, Daniel (1990). Structure of a mouse histone-encoding gene cluster. Gene, 95(2), pp. 303-304. Elsevier

Soldati, D; Schümperli, Daniel (1990). Structures of four human pseudogenes for U7 small nuclear RNA. Gene, 95(2), pp. 305-306. Elsevier

Meier, V S; Böhni, R; Schümperli, Daniel (1989). Nucleotide sequence of two mouse histone H4 genes. Nucleic acids research, 17(2), p. 795. Information Retrieval Ltd.

Book Section

Meyer, K.; Schümperli, Daniel (2012). Antisense Derivatives of U7 Small Nuclear RNA as Modulators of Pre-mRNA Splicing. In: Stamm, Stefan; Smith, Christopher W. J.; Lührmann, Reinhard (eds.) Alternative pre-mRNA Splicing: Theory and Protocols (pp. 481-494). Chichester: John Wiley & Sons 10.1002/9783527636778.ch45

Nlend Nlend, R.; Schümperli, Daniel (2012). Antisense Genes to Induce Exon Inclusion. In: Aartsma-Rus, Annemieke (ed.) Exon Skipping : Methods and Protocols. Methods in Molecular Biology: Vol. 867 (pp. 325-347). Heidelberg: Springer 10.1007/978-1-61779-767-5_21

Schümperli, Daniel (2012). Beeinflusst die moderne Bio- und Gentechnologie die Evolution? In: Dubach, Martina (ed.) Evolution wohin? Die Folgen der darwinschen Theorie (pp. 201-213). Zürich: vdf Hochschulverlag

Schümperli, Daniel (2012). Eigenschaften gentechnisch veränderter Pflanzen. In: , (ed.) Nutzen und Risiken der Freisetzung gentechnisch veränderter Pflanzen. Chancen nutzen, Risiken vermeiden, Kompetenzen erhalten. Programmsynthese NFP 59 (pp. 74-95). Zürich: vdf Hochschulverlag

Schümperli, Daniel (2002). Megatrends in medicine. In: Leuthold, M.; Leuenberger, H.; Weibel, E.R. (eds.) Megatrends – rise and fall of megatrends in science. Basel: Schwabe

Stauber, C; Soldati, D; Lüscher, B; Schümperli, Daniel (1990). Histone-specific RNA 3' processing in nuclear extracts from mammalian cells. In: RNA Processing Part B: Specific Methods. Methods in enzymology: Vol. 181 (pp. 74-89). San Diego, CA: Academic Press

Conference or Workshop Item

Duque, S.; Porensky, P.; William, D.A.; Odermatt, Philipp; Nlend, Rachel; Bevan, A.K.; Foust, K.; Braun, L.; Schmelzer, L.; Schümperli, Daniel; Kaspar, B.K.; Burghes, A.H.M. (2013). O.8 Intrathecal delivery of AAV9 vectors to model and rescue a large animal model of SMA. Neuromuscular disorders, 23(9-10), p. 797. Elsevier 10.1016/j.nmd.2013.06.558

Burghes, A.; McGovern, V.; Porensky, P.; Duque, S.; Bevan, A.; Foust, K.; Odermatt, P.; Nlend, R.; Massoni-Laporte, A.; Mitrpant, C.; Wilton, S.; Schümperli, Daniel; Kaspar, B. (October 2012). Spinal muscular atrophy SMA: SMNs spatial requirement and therapies. Neuromuscular disorders, 22(9-10), pp. 907-908. Amsterdam: Elsevier 10.1016/j.nmd.2012.06.342

Ruepp, Marc-David; Schweingruber, Christoph; Kleinschmidt, Nicole; Schümperli, Daniel (2010). Interactions of symplekin, CstF-64, and CstF-77. Implications for localisation, stability and function. In: The Fifteenth Annual Meeting of the RNA Society Seattle, Washington USA, June 22-26, 2010 (p. 1). RNA Society

Ittig, Damian; Schümperli, Daniel; Leumann, Christian J. (2008). Tc-DNA modified siRNA. Nucleic Acids Symposium Series, 52(1), pp. 501-502. Oxford University Press 10.1093/nass/nrn254

Schümperli, Daniel; Albrecht, U; Koning, T W; Melin, L; Soldati, D; Stauber, C; Lührmann, R (1990). Biochemical studies of U7 snRNPs and of histone RNA 3' processing. Molecular Biology Reports, 14(2-3), pp. 205-206. Kluwer Academic Publishers

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